Epitope Mapping pdf

Author:
Edition: 1
Publisher: Oxford University Press, USA
Binding: Paperback
ISBN: 0199636524

Epitope Mapping: A Practical Approach

Epitope Mapping covers all the major methods for the identification and definition of epitopes. Medical books Epitope Mapping. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotoxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning Medical books Epitope Mapping Protocols. Epitope Mapping Protocols: Mike Schutkowski, Ulrich Reineke

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Epitope Mapping Protocols: Mike Schutkowski, Ulrich Reineke

Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping.

EPITOPE MAPPING: A PRACTICAL APPROACH. The Internet Bookshop UK Ltd ISBN: 0199636524 Title: EPITOPE MAPPING: A PRACTICAL APPROACH. Author: Westwood, Olwyn MR & Frank C. Hay (edits). Publisher: OUP Publication Date: 2001 Binding: Paperback Condition: Very Good SKU:1649448 Description: Details: 2001. OUP. First. Softback. Book- VG. 9.5x7.5. 284pp. Some b/w illus. UK 3-5 working days. Mainland Europe And USA 7-10 working days

Epitope Mapping Protocols offers more than thirty protocols for antibody production and mapping -- all described in detail by leading experts and illustrated by proven examples from their own work. The methods range from those that use (or can use) whole, native antigens to those that use peptides and peptide libraries and those that require antigen expressed from recombinant DNA. Author: Morris, Glenn E. ISBN-10: 0896033759

Medical Book Epitope Mapping

The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotoxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific epitope. Investigation of carbohydrates can also be useful to epitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.